Ambrożkiewicz, Karolina A; Kozłowska, Urszula; Haesler, Valerie; Barnea, Eytan R; Müller, Martin; Kurpisz, Maciej (2022). Murine glial progenitor cells transplantation and synthetic PreImplantation Factor (sPIF) reduces inflammation and early motor impairment in ALS mice. Scientific Reports, 12(1), p. 4016. Nature Publishing Group 10.1038/s41598-022-08064-9
|
Text
s41598-022-08064-9.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (7MB) | Preview |
Amyotrophic lateral sclerosis (ALS) is a progressive motor neuronal disorder characterized by neuronal degeneration and currently no effective cure is available to stop or delay the disease from progression. Transplantation of murine glial-restricted precursors (mGRPs) is an attractive strategy to modulate ALS development and advancements such as the use of immune modulators could potentially extend graft survival and function. Using a well-established ALS transgenic mouse model (SOD1G93A), we tested mGRPs in combination with the immune modulators synthetic PreImplantation Factor (sPIF), Tacrolimus (Tac), and Costimulatory Blockade (CB). We report that transplantation of mGRPs into the cisterna magna did not result in increased mice survival. The addition of immunomodulatory regimes again did not increase mice lifespan but improved motor functions and sPIF was superior compared to other immune modulators. Immune modulators did not affect mGRPs engraftment significantly but reduced pro-inflammatory cytokine production. Finally, sPIF and CB reduced the number of microglial cells and prevented neuronal number loss. Given the safety profile and a neuroprotective potential of sPIF, we envision its clinical application in near future.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin |
UniBE Contributor: |
Müller, Martin (A) |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2045-2322 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
09 Mar 2022 10:51 |
Last Modified: |
29 Mar 2023 23:38 |
Publisher DOI: |
10.1038/s41598-022-08064-9 |
PubMed ID: |
35256767 |
Additional Information: |
These authors contributed equally: Martin Mueller and Maciej Kurpisz. |
BORIS DOI: |
10.48350/166893 |
URI: |
https://boris.unibe.ch/id/eprint/166893 |
Actions (login required)
 |
Edit item |