Dewar, Caroline E; Oeljeklaus, Silke; Wenger, Christoph; Warscheid, Bettina; Schneider, André (2022). Characterisation of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei. The journal of biological chemistry, 298(4), p. 101829. Elsevier 10.1016/j.jbc.2022.101829
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The mitochondrial F1Fo ATP synthase of the parasite Trypanosoma brucei has been previously studied in detail. This unusual enzyme switches direction in functionality during the life cycle of the parasite, acting as an ATP synthase in the insect stages, and as an ATPase to generate mitochondrial membrane potential in the mammalian bloodstream stages. Whereas the trypanosome F1 moiety is relatively highly conserved in structure and composition, the Fo subcomplex and the peripheral stalk have been shown to be more variable. Interestingly, a core subunit of the latter, the normally conserved subunit b, has been resistant to identification by sequence alignment or biochemical methods. Here we identified a 17 kDa mitochondrial protein of the inner membrane, Tb927.8.3070, that is essential for normal growth, efficient oxidative phosphorylation, and membrane potential maintenance. Pulldown experiments and native PAGE analysis indicated that the protein is both associated with the F1Fo ATP synthase, and integral to its assembly. In addition, its knockdown reduced the levels of Fo subunits, but not those of F1, and disturbed the cell cycle. Finally, analysis of structural homology using the HHpred algorithm showed that this protein has structural similarities to Fo subunit b of other species, indicating that this subunit may be a highly diverged form of the elusive subunit b.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Dewar, Caroline Elizabeth, Wenger, Christoph, Schneider, André |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1083-351X |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
17 Mar 2022 11:43 |
Last Modified: |
05 Dec 2022 16:15 |
Publisher DOI: |
10.1016/j.jbc.2022.101829 |
PubMed ID: |
35293314 |
Uncontrolled Keywords: |
ATP synthase Trypanosoma brucei mitochondria proteomics protozoan |
BORIS DOI: |
10.48350/167514 |
URI: |
https://boris.unibe.ch/id/eprint/167514 |
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