Platelet concentrates in platelet additive solutions generate less complement activation products during storage than platelets stored in plasma.

de Wit, Yasmin E S; Vlaar, Richard; Gouwerok, Eric; Hamzeh-Cognasse, Hind; van Mierlo, Gerard; Bulder, Ingrid; Lagerberg, Johan W M; de Korte, Dirk; Cognasse, Fabrice; Ten Brinke, Anja; Zeerleder, Sacha S (2023). Platelet concentrates in platelet additive solutions generate less complement activation products during storage than platelets stored in plasma. Blood transfusion, 21(2), pp. 157-167. SIMTI Servizi Srl 10.2450/2022.0323-21

[img] Text
323-21__Epub_ahead_of_print.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

BACKGROUND

Platelet transfusions can be associated with adverse reactions, such as febrile non-haemolytic transfusion reaction (FNHTR). It has been suggested that damage-associated molecular patterns (DAMP) and complement play a role in FNHTR. This study investigated the nature of DAMPs and complement activation products contained in platelet concentrates during storage, with a specific focus on different platelet storage solutions.

MATERIALS AND METHODS

Buffy coats (BC) from healthy donors were pooled (15 BC per pool) and divided into three groups of the same volume. After addition of different storage solutions (plasma, platelet additive solutions [PAS]-C or PAS-E; n=6 for each group), BC pools were processed to platelet concentrates (PC). Leukoreduced PCs were stored on a shaking bed at 20-24°C and sampled on days 1, 2, 6 and 8 after collection for selected quality parameters: platelet activation, DAMPs (High Mobility Group Box 1 [HMGB1], nucleosomes), and complement activation products.

RESULTS

During storage, equal levels of free nucleosomes and increasing concentrations of HMGB1 were present in all groups. Complement activation was observed in all PC. However, by day 8, the use of PAS had reduced C3b/c levels by approximately 90% and C4b/c levels by approximately 65%.

DISCUSSION

Nucleosomes and HMGB1 were present in PCs prepared in plasma and PAS. Complement was activated during storage of platelets in plasma and in PAS. The use of PAS is associated with a lower amount of complement activation products due to the dilution of plasma by PAS. Therefore, PC in PAS have less complement activation products than platelets stored in plasma. These proinflammatory mediators in PC might induce FNHTR.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Hämatologie (Erwachsene)

UniBE Contributor:

Zeerleder, Sacha Sergio

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1723-2007

Publisher:

SIMTI Servizi Srl

Language:

English

Submitter:

Pubmed Import

Date Deposited:

21 Mar 2022 09:35

Last Modified:

18 Mar 2023 00:11

Publisher DOI:

10.2450/2022.0323-21

PubMed ID:

35302481

BORIS DOI:

10.48350/167645

URI:

https://boris.unibe.ch/id/eprint/167645

Actions (login required)

Edit item Edit item
Provide Feedback