Secondary influenza challenge triggers resident memory B cell migration and rapid relocation to boost antibody secretion at infected sites.

MacLean, Andrew J; Richmond, Niamh; Koneva, Lada; Attar, Moustafa; Medina, Cesar A P; Thornton, Emily E; Gomes, Ariane Cruz; El-Turabi, Aadil; Bachmann, Martin F; Rijal, Pramila; Tan, Tiong Kit; Townsend, Alain; Sansom, Stephen N; Bannard, Oliver; Arnon, Tal I (2022). Secondary influenza challenge triggers resident memory B cell migration and rapid relocation to boost antibody secretion at infected sites. Immunity, 55(4), 718-733.e8. Cell Press 10.1016/j.immuni.2022.03.003

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Resident memory B (BRM) cells develop and persist in the lungs of influenza-infected mice and humans; however, their contribution to recall responses has not been defined. Here, we used two-photon microscopy to visualize BRM cells within the lungs of influenza -virus immune and reinfected mice. Prior to re-exposure, BRM cells were sparsely scattered throughout the tissue, displaying limited motility. Within 24 h of rechallenge, these cells increased their migratory capacity, localized to infected sites, and subsequently differentiated into plasma cells. Alveolar macrophages mediated this process, in part by inducing expression of chemokines CXCL9 and CXCL10 from infiltrating inflammatory cells. This led to the recruitment of chemokine receptor CXCR3-expressing BRM cells to infected regions and increased local antibody concentrations. Our study uncovers spatiotemporal mechanisms that regulate lung BRM cell reactivation and demonstrates their capacity to rapidly deliver antibodies in a highly localized manner to sites of viral replication.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology and Immunology

UniBE Contributor:

Bachmann, Martin (B)

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1074-7613

Publisher:

Cell Press

Language:

English

Submitter:

Pubmed Import

Date Deposited:

30 Mar 2022 09:12

Last Modified:

29 Mar 2023 23:38

Publisher DOI:

10.1016/j.immuni.2022.03.003

PubMed ID:

35349789

Uncontrolled Keywords:

antibody response humoral immunity influenza virus live imaging memory B cell mucosal immunity plasma cells resident memory B cells tissue-resident immunity two-photon microscopy

BORIS DOI:

10.48350/168405

URI:

https://boris.unibe.ch/id/eprint/168405

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