Leaf Asymmetry as a Developmental Constraint Imposed by Auxin-Dependent Phyllotactic Patterning

Chitwood, Daniel H; Headland, Lauren R; Ranjan, Ashish; Martinez, Ciera C; Braybrook, Siobhan; Koenig, Daniel P; Kuhlemeier, Cris; Smith, Richard Simon; Sinha, Neelima R (2012). Leaf Asymmetry as a Developmental Constraint Imposed by Auxin-Dependent Phyllotactic Patterning. The Plant Cell, 24(6), pp. 2318-2327. Rockville, Md.: American Society of Plant Biologists 10.​1105/​tpc.​112.​098798

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In a majority of species, leaf development is thought to proceed in a bilaterally symmetric fashion without systematic asymmetries. This is despite the left and right sides of an initiating primordium occupying niches that differ in their distance from sinks and sources of auxin. Here, we revisit an existing model of auxin transport sufficient to recreate spiral phyllotactic patterns and find previously overlooked asymmetries between auxin distribution and the centers of leaf primordia. We show that it is the direction of the phyllotactic spiral that determines the side of the leaf these asymmetries fall on. We empirically confirm the presence of an asymmetric auxin response using a DR5 reporter and observe morphological asymmetries in young leaf primordia. Notably, these morphological asymmetries persist in mature leaves, and we observe left-right asymmetries in the superficially bilaterally symmetric leaves of tomato (Solanum lycopersicum) and Arabidopsis thaliana that are consistent with modeled predictions. We further demonstrate that auxin application to a single side of a leaf primordium is sufficient to recapitulate the asymmetries we observe. Our results provide a framework to study a previously overlooked developmental axis and provide insights into the developmental constraints imposed upon leaf morphology by auxin-dependent phyllotactic patterning.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development

UniBE Contributor:

Braybrook, Siobhan; Kuhlemeier, Cris and Smith, Richard Simon

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1040-4651

Publisher:

American Society of Plant Biologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

04 Oct 2013 14:41

Last Modified:

31 Mar 2017 11:40

Publisher DOI:

10.​1105/​tpc.​112.​098798

Web of Science ID:

000306919300010

BORIS DOI:

10.7892/boris.16908

URI:

https://boris.unibe.ch/id/eprint/16908 (FactScience: 224614)

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