Kargarpour, Zahra; Panahipour, Layla; Miron, Richard J; Gruber, Reinhard (2022). Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols. Molecules, 27(7) Molecular Diversity Preservation International MDPI 10.3390/molecules27072043
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Liquid platelet-rich fibrin (PRF) is produced by fractionation of blood without additives that initiate coagulation. Even though liquid PRF is frequently utilized as a natural source of fibrinogen to prepare sticky bone, the concentration of fibrinogen and the overall amount of "clottable PRF" components have not been evaluated. To this aim, we prepared liquid PRF at 300, 700, and 2000 relative centrifugal force (RCF), for 8 min and quantified the fibrinogen levels by immunoassay. We report here that, independent of the RCF, the fibrinogen concentration is higher in the platelet-poor plasma (PPP) compared to the buffy coat (BC) fraction of liquid PRF and further decreases in the remaining red fraction. We then determined the weight of the clotted PRF fractions before and after removing the serum. The PPP and BC fractions consist of 10.2% and 25.3% clottable matrix suggesting that more than half of the weight of clottable BC is caused by cellular components. Our data provide insights into the distribution of fibrinogen in the different fractions of liquid PRF. These findings suggest that PPP is the main source of clottable fibrinogen, while the BC is more a cell source when it comes to the preparation of sticky bone.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Miron, Richard John, Gruber, Reinhard |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1420-3049 |
Publisher: |
Molecular Diversity Preservation International MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
13 Apr 2022 13:57 |
Last Modified: |
05 Dec 2022 16:18 |
Publisher DOI: |
10.3390/molecules27072043 |
PubMed ID: |
35408442 |
Uncontrolled Keywords: |
blood clot blood coagulation buffy coat fibrinogen platelet-poor plasma platelet-rich fibrin sticky bone |
BORIS DOI: |
10.48350/169280 |
URI: |
https://boris.unibe.ch/id/eprint/169280 |
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