Peruzzu, Angela; Mastrandrea, Scilla; Fancellu, Alessandro; Bonelli, Piero; Muehlethaler, Konrad; Masala, Giovanna; Santucciu, Cinzia (2022). Comparison and evaluation of analytic and diagnostic performances of four commercial kits for the detection of antibodies against Echinococcus granulosus and multilocularis in human sera. Comparative immunology, microbiology and infectious diseases, 86, p. 101816. Elsevier 10.1016/j.cimid.2022.101816
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Cystic echinococcosis (CE) is a disease caused by Echinococcus granulosus sensu lato (s.l.), an ubiquitous worldwide zoonotic agent affecting humans and animals. Diagnosis of CE in humans is usually performed by imagine techniques along with immunoassays. The aim of our study was to evaluate and compare four commercial diagnostic kits, based on the detection of IgG antibodies against E. granulosus and E. multilocularis. The study was performed on a total of 259 sera: the positive (n = 74) and the negative (n = 185) group. The following analytic and diagnostic performances of the four kits were evaluated: operator skills, specificity, sensitivity, repeatability, reproducibility, accuracy, positive and negative predictive values. Based on the parameters evaluated, all four tests demonstrated excellent quality and proved to be reliable diagnostic tools to support the clinical evaluation of human patients suspected of having CE. The four commercial assays, in our hands, presented altogether, a range of performances from good to excellent, being immunoblotting (IB) the most reliable, used as gold standard, followed by the immunochromatographic test (ICT) and finally the two enzyme linked immunosorbent assay (ELISAs).
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases |
UniBE Contributor: |
Mühlethaler, Konrad |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0147-9571 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
27 Apr 2022 10:37 |
Last Modified: |
05 Dec 2022 16:19 |
Publisher DOI: |
10.1016/j.cimid.2022.101816 |
PubMed ID: |
35472655 |
Uncontrolled Keywords: |
Analytic and diagnostic performances Cystic echinococcosis Diagnostic techniques Echinococcus granulosus sensu stricto Immunoassays Management of the quality system Serodiagnosis |
BORIS DOI: |
10.48350/169543 |
URI: |
https://boris.unibe.ch/id/eprint/169543 |
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