An RNA-sequencing transcriptome of the rodent Schwann cell response to peripheral nerve injury.

Brosius Lutz, Amanda; Lucas, Tawaun A; Carson, Glenn A; Caneda, Christine; Zhou, Lu; Barres, Ben A; Buckwalter, Marion S; Sloan, Steven A (2022). An RNA-sequencing transcriptome of the rodent Schwann cell response to peripheral nerve injury. Journal of neuroinflammation, 19(1), p. 105. BioMed Central 10.1186/s12974-022-02462-6

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BACKGROUND

The important contribution of glia to mechanisms of injury and repair of the nervous system is increasingly recognized. In stark contrast to the central nervous system (CNS), the peripheral nervous system (PNS) has a remarkable capacity for regeneration after injury. Schwann cells are recognized as key contributors to PNS regeneration, but the molecular underpinnings of the Schwann cell response to injury and how they interact with the inflammatory response remain incompletely understood.

METHODS

We completed bulk RNA-sequencing of Schwann cells purified acutely using immunopanning from the naïve and injured rodent sciatic nerve at 3, 5, and 7 days post-injury. We used qRT-PCR and in situ hybridization to assess cell purity and probe dataset integrity. Finally, we used bioinformatic analysis to probe Schwann cell-specific injury-induced modulation of cellular pathways.

RESULTS

Our data confirm Schwann cell purity and validate RNAseq dataset integrity. Bioinformatic analysis identifies discrete modules of genes that follow distinct patterns of regulation in the 1st days after injury and their corresponding molecular pathways. These findings enable improved differentiation of myeloid and glial components of neuroinflammation after peripheral nerve injury and highlight novel molecular aspects of the Schwann cell injury response such as acute downregulation of the AGE/RAGE pathway and of secreted molecules Sparcl1 and Sema5a.

CONCLUSIONS

We provide a helpful resource for further deciphering the Schwann cell injury response and a depth of transcriptional data that can complement the findings of recent single cell sequencing approaches. As more data become available on the response of CNS glia to injury, we anticipate that this dataset will provide a valuable platform for understanding key differences in the PNS and CNS glial responses to injury and for designing approaches to ameliorate CNS regeneration.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin

UniBE Contributor:

Brosius Lutz, Amanda

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1742-2094

Publisher:

BioMed Central

Language:

English

Submitter:

Pubmed Import

Date Deposited:

04 May 2022 09:31

Last Modified:

05 Dec 2022 16:19

Publisher DOI:

10.1186/s12974-022-02462-6

PubMed ID:

35501870

Uncontrolled Keywords:

Injury response Macrophage Neuroinflammation Peripheral nerve Regeneration Repair cell Schwann cell Transcriptome

BORIS DOI:

10.48350/169716

URI:

https://boris.unibe.ch/id/eprint/169716

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