Genome Engineering of the Fast-Growing Mycoplasma feriruminatoris toward a Live Vaccine Chassis.

Talenton, Vincent; Baby, Vincent; Gourgues, Geraldine; Mouden, Charlotte; Claverol, Stephane; Vashee, Sanjay; Blanchard, Alain; Labroussaa, Fabien; Jores, Joerg; Arfi, Yonathan; Sirand-Pugnet, Pascal; Lartigue, Carole (2022). Genome Engineering of the Fast-Growing Mycoplasma feriruminatoris toward a Live Vaccine Chassis. ACS synthetic biology, 11(5), pp. 1919-1930. American Chemical Society 10.1021/acssynbio.2c00062

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Development of a new generation of vaccines is a key challenge for the control of infectious diseases affecting both humans and animals. Synthetic biology methods offer new ways to engineer bacterial chassis that can be used as vectors to present heterologous antigens and train the immune system against pathogens. Here, we describe the construction of a bacterial chassis based on the fast-growing Mycoplasma feriruminatoris, and the first steps toward its application as a live vaccine against contagious caprine pleuropneumonia (CCPP). To do so, the M. feriruminatoris genome was cloned in yeast, modified by iterative cycles of Cas9-mediated deletion of loci encoding virulence factors, and transplanted back in Mycoplasma capricolum subsp. capricolum recipient cells to produce the designed M. feriruminatoris chassis. Deleted genes encoded the glycerol transport and metabolism systems GtsABCD and GlpOKF and the Mycoplasma Ig binding protein-Mycoplasma Ig protease (MIB-MIP) immunoglobulin cleavage system. Phenotypic assays of the M. feriruminatoris chassis confirmed the corresponding loss of H2O2 production and IgG cleavage activities, while growth remained unaltered. The resulting mycoplasma chassis was further evaluated as a platform for the expression of heterologous surface proteins. A genome locus encoding an inactivated MIB-MIP system from the CCPP-causative agent Mycoplasma capricolum subsp. capripneumoniae was grafted in replacement of its homolog at the original locus in the chassis genome. Both heterologous proteins were detected in the resulting strain using proteomics, confirming their expression. This study demonstrates that advanced genome engineering methods are henceforth available for the fast-growing M. feriruminatoris, facilitating the development of novel vaccines, in particular against major mycoplasma diseases.

Item Type:

Journal Article (Original Article)


05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Labroussaa, Fabien, Jores, Jörg


600 Technology > 630 Agriculture




American Chemical Society




Pubmed Import

Date Deposited:

06 May 2022 09:56

Last Modified:

05 Dec 2022 16:19

Publisher DOI:


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Uncontrolled Keywords:

CRISPR-Cas9 CReasPy-cloning Mycoplasma feriruminatoris bacterial chassis contagious caprine pleuropneumonia genome engineering genome transplantation synthetic biology vaccine




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