Weusmann, Jens; Deschner, James; Imber, Jean-Claude; Damanaki, Anna; Cerri, Paulo Sérgio; Da Ponte Leguizamón, Natalia; Beisel-Memmert, Svenja; Nogueira, Andressa Vilas Boas (2022). Impact of glycine and erythritol/chlorhexidine air-polishing powders on human gingival fibroblasts: an in vitro study. Annals of anatomy - Anatomischer Anzeiger, 243, p. 151949. Elsevier 10.1016/j.aanat.2022.151949
|
Text
1-s2.0-S0940960222000644-main.pdf - Accepted Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (1MB) | Preview |
BACKGROUND
Supra- and subgingival air-polishing has been used in periodontitis and gingivitis therapy for years. Low-abrasive types of powders have facilitated the application in subgingival areas. In this study, the cellular effects of a glycine powder and an erythritol/chlorhexidine (CHX) powder on human gingival fibroblasts (HGF) were investigated.
METHODS
HGF were obtained from sound gingiva of three healthy donors. After 12hours and 24hours of incubation time, cell viability testing and, after 24hours and 48hours, a cell proliferation assay was conducted. Additionally, the individual components erythritol and CHX were investigated for cell viability. In vitro wound healing was monitored for 48hours and scanning electron microscopy (SEM) analysis was performed after 24hours. Statistical analysis was accomplished by ANOVA and post hoc Dunnett's and Tukey's tests (p < 0.05) were performed.
RESULTS
Erythritol/CHX powder and in a lower extent, glycine powder decreased cell viability and cell proliferation. The negative effect of erythritol/CHX was mainly based on the CHX component. In vitro wound healing was negatively influenced in both types of powders compared to control. Cell size was altered in both test groups, whereas cell morphology was affected only in the erythritol/CHX group.
CONCLUSIONS
The investigated powders for subgingival air-polishing can influence cell viability, morphology, and proliferation, as well as wound closure in vitro. These actions on fibroblasts are discernible, with the cytotoxic effect of erythritol/CHX powder being very clear and mainly due to the CHX component. Our results suggest that subgingivally applied powders can exert direct effects on gingival fibroblasts.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Imber, Jean-Claude |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0940-9602 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
09 May 2022 09:28 |
Last Modified: |
06 May 2023 00:25 |
Publisher DOI: |
10.1016/j.aanat.2022.151949 |
PubMed ID: |
35523398 |
Uncontrolled Keywords: |
air polishing – periodontology – cell biology – glycine – erythritol – chlorhexidine |
BORIS DOI: |
10.48350/169835 |
URI: |
https://boris.unibe.ch/id/eprint/169835 |
Actions (login required)
 |
Edit item |