Multistep enzyme cascades as a route towards green and sustainable pharmaceutical syntheses.

Benítez-Mateos, Ana I; Roura Padrosa, David; Paradisi, Francesca (2022). Multistep enzyme cascades as a route towards green and sustainable pharmaceutical syntheses. Nature chemistry, 14(5), pp. 489-499. Springer Nature 10.1038/s41557-022-00931-2

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Enzyme cascades are a powerful technology to develop environmentally friendly and cost-effective synthetic processes to manufacture drugs, as they couple different biotransformations in sequential reactions to synthesize the product. These biocatalytic tools can address two key parameters for the pharmaceutical industry: an improved selectivity of synthetic reactions and a reduction of potential hazards by using biocompatible catalysts, which can be produced from sustainable sources, which are biodegradable and, generally, non-toxic. Here we outline a broad variety of enzyme cascades used either in vivo (whole cells) or in vitro (purified enzymes) to specifically target pharmaceutically relevant molecules, from simple building blocks to complex drugs. We also discuss the advantages and requirements of multistep enzyme cascades and their combination with chemical catalysts through a series of reported examples. Finally, we examine the efficiency of enzyme cascades and how they can be further improved by enzyme engineering, process intensification in flow reactors and/or enzyme immobilization to meet all the industrial requirements.

Item Type:

Journal Article (Review Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

UniBE Contributor:

Benitez Mateos, Ana Isabel, Roura Padrosa, David, Paradisi, Francesca

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1755-4349

Publisher:

Springer Nature

Language:

English

Submitter:

Pubmed Import

Date Deposited:

09 May 2022 09:38

Last Modified:

05 Dec 2022 16:19

Publisher DOI:

10.1038/s41557-022-00931-2

PubMed ID:

35513571

BORIS DOI:

10.48350/169847

URI:

https://boris.unibe.ch/id/eprint/169847

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