Quantification of NAD+ in human brain with 1 H MR spectroscopy at 3 T: Comparison of three localization techniques with different handling of water magnetization.

Dziadosz, Martyna; Hoefemann, Maike; Döring, André; Marjańska, Malgorzata; Auerbach, Edward John; Kreis, Roland (2022). Quantification of NAD+ in human brain with 1 H MR spectroscopy at 3 T: Comparison of three localization techniques with different handling of water magnetization. Magnetic resonance in medicine, 88(3), pp. 1027-1038. Wiley-Liss 10.1002/mrm.29267

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PURPOSE

The detection of nicotinamide-adenine-dinucleotide (NAD+ ) is challenging using standard 1 H MR spectroscopy, because it is of low concentration and affected by polarization-exchange with water. Therefore, this study compares three techniques to access NAD+ quantification at 3 T-one with and two without water presaturation.

METHODS

A large brain volume in 10 healthy subjects was investigated with three techniques: semi-LASER with water-saturation (WS) (TE = 35 ms), semi-LASER with metabolite-cycling (MC) (TE = 35 ms), and the non-water-excitation (nWE) technique 2D ISIS-localization with chemical-shift-selective excitation (2D I-CSE) (TE = 10.2 ms). Spectra were quantified with optimized modeling in FiTAID.

RESULTS

NAD+ could be well quantified in cohort-average spectra with all techniques. Obtained apparent NAD+ tissue contents are all lower than expected from literature confirming restricted visibility by 1 H MRS. The estimated value from WS-MRS (58 μM) was considerably lower than those obtained with non-WS techniques (146 μM for MC-semi-LASER and 125 μM for 2D I-CSE). The nWE technique with shortest TE gave largest NAD+ signals but suffered from overlap with large amide signals. MC-semi-LASER yielded best estimation precision as reflected in relative Cramer-Rao bounds (14%, 21 μM/146 μM) and also best robustness as judged by the coefficient-of-variance over the cohort (11%, 10 μM/146 μM). The MR-visibility turned out as 16% with WS and 41% with MC.

CONCLUSION

Three methods to assess NAD+ in human brain at 3 T have been compared. NAD+ could be detected with a visibility of ∼41% for the MC method. This may open a new window for the observation of pathological changes in the clinical research setting.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Abt. Magnetresonanz-Spektroskopie und Methodologie, AMSM
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic and Interventional Neuroradiology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Dziadosz, Martyna; Höfemann, Maike Svenja and Kreis, Roland

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0740-3194

Publisher:

Wiley-Liss

Language:

English

Submitter:

Pubmed Import

Date Deposited:

10 May 2022 10:04

Last Modified:

30 Jun 2022 00:14

Publisher DOI:

10.1002/mrm.29267

PubMed ID:

35526238

Uncontrolled Keywords:

MR spectroscopy NAD+ brain detectability fitting magnetization exchange modeling precision quantification saturation transfer

BORIS DOI:

10.48350/169882

URI:

https://boris.unibe.ch/id/eprint/169882

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