Dommann, Noëlle; Gavini, Jacopo; Sánchez-Taltavull, Daniel; Baier, Felix Alexander; Birrer, Fabienne; Loforese, Giulio; Candinas, Daniel; Stroka, Deborah (2022). LIM protein Ajuba promotes liver cell proliferation through its involvement in DNA replication and DNA damage control. FEBS letters, 596(14), pp. 1746-1764. FEBS Press 10.1002/1873-3468.14371
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FEBS_Letters_-_2022_-_Dommann_-_LIM_protein_Ajuba_promotes_liver_cell_proliferation_through_its_involvement_in_DNA.pdf - Accepted Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (15MB) | Preview |
The LIM-domain protein Ajuba is associated with cell proliferation, a fundamental process of tissue regeneration and cancer. We report that in the liver, Ajuba expression is increased during regeneration and in tumor cells and tissues. Knockout of Ajuba using CRISPR/Cas9 is embryonic lethal in mice. shRNA targeting of Ajuba reduces cell proliferation, delays cell entry into S-phase, reduces cell survival and tumor growth in vivo, and increases expression of the DNA damage marker γH2AX. Ajuba binding partners include proteins involved in DNA replication and damage, such as SKP2, MCM2, MCM7 and RPA70. Taken together, our data support that Ajuba promotes liver cell proliferation associated with development, regeneration, and tumor growth and is involved in DNA replication and damage repair.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine |
UniBE Contributor: |
Dommann, Noëlle Annick, Gavini, Jacopo, Sánchez Taltavull, Daniel, Baier, Felix Alexander, Birrer, Fabienne Esther, Loforese, Giulio, Candinas, Daniel, Stroka, Deborah |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1873-3468 |
Publisher: |
FEBS Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
11 May 2022 09:35 |
Last Modified: |
05 Dec 2022 16:19 |
Publisher DOI: |
10.1002/1873-3468.14371 |
PubMed ID: |
35535434 |
BORIS DOI: |
10.48350/169914 |
URI: |
https://boris.unibe.ch/id/eprint/169914 |
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