Corynebacterium uberis sp. nov. frequently isolated from bovine mastitis.

Kittl, Sonja; Studer, Eveline; Brodard, Isabelle; Thomann, Andreas; Jores, Jörg (2022). Corynebacterium uberis sp. nov. frequently isolated from bovine mastitis. Systematic and applied microbiology, 45(4), p. 126325. Elsevier 10.1016/j.syapm.2022.126325

[img]
Preview
Text
1-s2.0-S0723202022000327-main.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (4MB) | Preview

Several strains belonging to the genus Corynebacterium, but not to any described species of the genus were isolated from bovine mastitic milk samples over the past five years in the diagnostic unit of the University of Bern. Six of these strains (18M0132T, 17M2518, 18M0913, 19M0083, 20M1046 and 20M1090) that were phenotypically similar were further characterized genotypically. Gram-positive coryneform rods were catalase positive, facultative anaerobe and CAMP-test negative. Whole genome sequencing and subsequent phylogenetic analysis revealed their genome size to be 2.53 Mb and their G + C content to be between 65.4 and 65.5 mol%. Digital DNA-DNA hybridisation (dDDH) showed the highest similarity of only less than 20% with Corynebacterium mastitidis and Corynebacterium frankenforstense, which indicated that the isolates belong to an undescribed Corynebacterium species. This was confirmed by studying the average nucleotide identity (ANI) where the accepted species boundary is around 95% and which ranged between 70.3% and 74.9% with the most closely related species C. mastitidis. We established MALDI-TOF fingerprints of the species, which allows a clear separation from related species and can be used by other laboratories for diagnostic purposes. Based on our analyses we conclude that the selected strains belong to a previously undescribed species and propose the name Corynebacterium uberis sp. nov. The proposed type strain is 18M0132T (=DSM 111922T, = CCOS 1972T).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants

UniBE Contributor:

Kittl, Sonja Cornelia; Studer, Eveline; Brodard, Isabelle; Thomann, Andreas and Jores, Jörg

Subjects:

600 Technology > 630 Agriculture

ISSN:

0723-2020

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

23 May 2022 12:17

Last Modified:

16 Jul 2022 00:14

Publisher DOI:

10.1016/j.syapm.2022.126325

PubMed ID:

35594686

Uncontrolled Keywords:

Bovine mastitis Corynebacteriaceae Corynebacterium uberis sp. nov. Phylogeny

BORIS DOI:

10.48350/170147

URI:

https://boris.unibe.ch/id/eprint/170147

Actions (login required)

Edit item Edit item
Provide Feedback