Small intestinal resident eosinophils maintain gut homeostasis following microbial colonization.

Ignacio, Aline; Shah, Kathleen; Bernier-Latmani, Jeremiah; Köller, Yasmin; Coakley, Gillian; Moyat, Mati; Hamelin, Romain; Armand, Florence; Wong, Nick C; Ramay, Hena; Thomson, Carolyn A; Burkhard, Regula; Wang, Haozhe; Dufour, Antoine; Geuking, Markus B; McDonald, Braedon; Petrova, Tatiana V; Harris, Nicola L; McCoy, Kathy D (2022). Small intestinal resident eosinophils maintain gut homeostasis following microbial colonization. Immunity, 55(7), 1250-1267.e12. Elsevier 10.1016/j.immuni.2022.05.014

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The intestine harbors a large population of resident eosinophils, yet the function of intestinal eosinophils has not been explored. Flow cytometry and whole-mount imaging identified eosinophils residing in the lamina propria along the length of the intestine prior to postnatal microbial colonization. Microscopy, transcriptomic analysis, and mass spectrometry of intestinal tissue revealed villus blunting, altered extracellular matrix, decreased epithelial cell turnover, increased gastrointestinal motility, and decreased lipid absorption in eosinophil-deficient mice. Mechanistically, intestinal epithelial cells released IL-33 in a microbiota-dependent manner, which led to eosinophil activation. The colonization of germ-free mice demonstrated that eosinophil activation in response to microbes regulated villous size alterations, macrophage maturation, epithelial barrier integrity, and intestinal transit. Collectively, our findings demonstrate a critical role for eosinophils in facilitating the mutualistic interactions between the host and microbiota and provide a rationale for the functional significance of their early life recruitment in the small intestine.

Item Type:

 Journal Article (Original Article)

Division/Institute:

 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie

UniBE Contributor:

 Köller, Yasmin

Subjects:

 600 Technology > 610 Medicine & health

ISSN:

 1097-4180

Publisher:

 Elsevier

Language:

 English

Submitter:

 Pubmed Import

Date Deposited:

 22 Jun 2022 15:13

Last Modified:

 05 Dec 2022 16:21

Publisher DOI:

 10.1016/j.immuni.2022.05.014

PubMed ID:

 35709757

Uncontrolled Keywords:

 eosinophil extracellular matrix germ-free intestinal barrier microbiome small intestine tissue homeostasis villous atrophy

BORIS DOI:

 10.48350/170784

URI:

 https://boris.unibe.ch/id/eprint/170784

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