A Metabolomic Analysis of Cirrhotic Ascites.

Beyoğlu, Diren; Simillion, Cedric; Storni, Federico; De Gottardi, Andrea; Idle, Jeffrey R (2022). A Metabolomic Analysis of Cirrhotic Ascites. Molecules, 27(12), p. 3935. MDPI 10.3390/molecules27123935

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Ascites is a common complication of decompensated liver cirrhosis, and yet relatively little is known about its biochemical composition. We conducted two metabolomic investigations, comparing the profile of ascites from 33 cirrhotic patients and postoperative peritoneal drainage fluid from 33 surgical patients (Experiment 1). The profile of paired ascites and plasma was also compared in 17 cirrhotic patients (Experiment 2). Gas chromatography-mass spectrometry-based metabolomics identified 29 metabolites that significantly characterized ascites fluid, whether postoperative drainage fluid or plasma were used as controls. Ten elevated amino acids (glutamine, proline, histidine, tyrosine, glycine, valine, threonine, methionine, lysine, phenylalanine) and seven diminished lipids (laurate, myristate, palmitate, oleate, vaccenate, stearate, cholesterol) largely comprised the cirrhotic ascites metabolomic phenotype that differed significantly (adjusted p < 0.002 to 0.03) from peritoneal drainage fluid or plasma. The pattern of upregulated amino acids in cirrhotic ascites did not indicate albumin proteolysis by peritoneal bacteria. Bidirectional clustering showed that the more severe the cirrhosis, the lower the lipid concentration in ascitic fluid. The metabolomic compartment of ascites in patients with decompensated cirrhosis is characterized by increased amino acids and decreased lipids. These novel findings have potential relevance for diagnostic purposes.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Hepatologie

UniBE Contributor:

Beyoglu, Diren, Storni, Federico Lorenzo

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1420-3049

Publisher:

MDPI

Language:

English

Submitter:

Pubmed Import

Date Deposited:

27 Jun 2022 08:33

Last Modified:

07 Aug 2024 15:45

Publisher DOI:

10.3390/molecules27123935

PubMed ID:

35745058

Uncontrolled Keywords:

amino acids ascites cholesterol decompensated cirrhosis fatty acids metabolomics

BORIS DOI:

10.48350/170904

URI:

https://boris.unibe.ch/id/eprint/170904

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