Zauhar, Randy; Biber, Josef; Jabri, Yassin; Kim, Mijin; Hu, Jian; Kaplan, Lew; Pfaller, Anna M; Schäfer, Nicole; Enzmann, Volker; Schlötzer-Schrehardt, Ursula; Straub, Tobias; Hauck, Stefanie M; Gamlin, Paul D; McFerrin, Michael B; Messinger, Jeffrey; Strang, Christianne E; Curcio, Christine A; Dana, Nicholas; Pauly, Diana; Grosche, Antje; ... (2022). As in Real Estate, Location Matters: Cellular Expression of Complement Varies Between Macular and Peripheral Regions of the Retina and Supporting Tissues. Frontiers in immunology, 13, p. 895519. Frontiers Research Foundation 10.3389/fimmu.2022.895519
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The cellular events that dictate the initiation of the complement pathway in ocular degeneration, such as age-related macular degeneration (AMD), is poorly understood. Using gene expression analysis (single cell and bulk), mass spectrometry, and immunohistochemistry, we dissected the role of multiple retinal and choroidal cell types in determining the complement homeostasis. Our scRNA-seq data show that the cellular response to early AMD is more robust in the choroid, particularly in fibroblasts, pericytes and endothelial cells. In late AMD, complement changes were more prominent in the retina especially with the expression of the classical pathway initiators. Notably, we found a spatial preference for these differences. Overall, this study provides insights into the heterogeneity of cellular responses for complement expression and the cooperation of neighboring cells to complete the pathway in healthy and AMD eyes. Further, our findings provide new cellular targets for therapies directed at complement.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde 04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology |
UniBE Contributor: |
Enzmann, Volker |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1664-3224 |
Publisher: |
Frontiers Research Foundation |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
06 Jul 2022 09:58 |
Last Modified: |
05 Dec 2022 16:21 |
Publisher DOI: |
10.3389/fimmu.2022.895519 |
PubMed ID: |
35784369 |
Uncontrolled Keywords: |
RPE/choroid age-related macular degeneration complement retina single cell |
BORIS DOI: |
10.48350/171128 |
URI: |
https://boris.unibe.ch/id/eprint/171128 |
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