A mRNA Vaccine Encoding for a RBD 60-mer Nanoparticle Elicits Neutralizing Antibodies and Protective Immunity Against the SARS-CoV-2 Delta Variant in Transgenic K18-hACE2 Mice.

Brandys, Pascal; Montagutelli, Xavier; Merenkova, Irena; Barut, Güliz T; Thiel, Volker; Schork, Nicholas J; Trüeb, Bettina; Conquet, Laurine; Deng, Aihua; Antanasijevic, Aleksandar; Lee, Hyun-Ku; Valière, Martine; Sindhu, Anoop; Singh, Gita; Herold, Jens (2022). A mRNA Vaccine Encoding for a RBD 60-mer Nanoparticle Elicits Neutralizing Antibodies and Protective Immunity Against the SARS-CoV-2 Delta Variant in Transgenic K18-hACE2 Mice. Frontiers in immunology, 13(912898), p. 912898. Frontiers Research Foundation 10.3389/fimmu.2022.912898

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Two years into the COVID-19 pandemic there is still a need for vaccines to effectively control the spread of novel SARS-CoV-2 variants and associated cases of severe disease. Here we report a messenger RNA vaccine directly encoding for a nanoparticle displaying 60 receptor binding domains (RBDs) of SARS-CoV-2 that acts as a highly effective antigen. A construct encoding the RBD of the Delta variant elicits robust neutralizing antibody response, and also provides protective immunity against the Delta variant in a widely used transgenic mouse model. We ultimately find that the proposed mRNA RBD nanoparticle-based vaccine provides a flexible platform for rapid development and will likely be of great value in combatting current and future SARS-CoV-2 variants of concern.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Barut, Güliz Tuba, Thiel, Volker Earl, Trüeb, Bettina Salome

Subjects:

600 Technology > 630 Agriculture

ISSN:

1664-3224

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Pubmed Import

Date Deposited:

27 Jul 2022 11:32

Last Modified:

05 Dec 2022 16:22

Publisher DOI:

10.3389/fimmu.2022.912898

PubMed ID:

35874687

Uncontrolled Keywords:

K18-hACE2 SARS-CoV-2 delta variant mRNA vaccine nanoparticle neutralizing antibody receptor-binding domain

BORIS DOI:

10.48350/171536

URI:

https://boris.unibe.ch/id/eprint/171536

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