Optimized intramuscular immunization with VSV-vectored spike protein triggers a superior immune response to SARS-CoV-2.

Taddeo, Adriano; Berenguer Veiga, Inês; Devisme, Christelle; Boss, Renate; Plattet, Philippe; Weigang, Sebastian; Kochs, Georg; Thiel, Volker; Benarafa, Charaf; Zimmer, Gert (2022). Optimized intramuscular immunization with VSV-vectored spike protein triggers a superior immune response to SARS-CoV-2. NPJ vaccines, 7(1), p. 82. Springer Nature 10.1038/s41541-022-00508-7

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Immunization with vesicular stomatitis virus (VSV)-vectored COVID-19 vaccine candidates expressing the SARS-CoV-2 spike protein in place of the VSV glycoprotein relies implicitly on expression of the ACE2 receptor at the muscular injection site. Here, we report that such a viral vector vaccine did not induce protective immunity following intramuscular immunization of K18-hACE2 transgenic mice. However, when the viral vector was trans-complemented with the VSV glycoprotein, intramuscular immunization resulted in high titers of spike-specific neutralizing antibodies. The vaccinated animals were fully protected following infection with a lethal dose of SARS-CoV-2-SD614G via the nasal route, and partially protected if challenged with the SARS-CoV-2Delta variant. While dissemination of the challenge virus to the brain was completely inhibited, replication in the lung with consequent lung pathology was not entirely controlled. Thus, intramuscular immunization was clearly enhanced by trans-complementation of the VSV-vectored vaccines by the VSV glycoprotein and led to protection from COVID-19, although not achieving sterilizing immunity.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Taddeo, Adriano; Berenguer Veiga, Inês Margarida; Devisme, Christelle; Plattet, Philippe; Thiel, Volker Earl; Benarafa, Charaf and Zimmer, Gert

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health

ISSN:

2059-0105

Publisher:

Springer Nature

Language:

English

Submitter:

Pubmed Import

Date Deposited:

27 Jul 2022 11:03

Last Modified:

05 Dec 2022 16:22

Publisher DOI:

10.1038/s41541-022-00508-7

PubMed ID:

35879345

BORIS DOI:

10.48350/171574

URI:

https://boris.unibe.ch/id/eprint/171574

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