Alterations in TRN-anterodorsal thalamocortical circuits affect sleep architecture and homeostatic processes in oxidative stress vulnerable Gclm-/- mice.

Czekus, Christina; Steullet, Pascal; Orero López, Albert; Bozic, Ivan; Rusterholz, Thomas; Bandarabadi, Mojtaba; Do, Kim Q; Gutierrez Herrera, Carolina (2022). Alterations in TRN-anterodorsal thalamocortical circuits affect sleep architecture and homeostatic processes in oxidative stress vulnerable Gclm-/- mice. Molecular psychiatry, 27(11), pp. 4394-4406. Springer Nature 10.1038/s41380-022-01700-w

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Schizophrenia is associated with alterations of sensory integration, cognitive processing and both sleep architecture and sleep oscillations in mouse models and human subjects, possibly through changes in thalamocortical dynamics. Oxidative stress (OxS) damage, including inflammation and the impairment of fast-spiking gamma-aminobutyric acid neurons have been hypothesized as a potential mechanism responsible for the onset and development of schizophrenia. Yet, the link between OxS and perturbation of thalamocortical dynamics and sleep remains unclear. Here, we sought to investigate the effects of OxS on sleep regulation by characterizing the dynamics of thalamocortical networks across sleep-wake states in a mouse model with a genetic deletion of the modifier subunit of glutamate-cysteine ligase (Gclm knockout, KO) using high-density electrophysiology in freely-moving mice. We found that Gcml KO mice exhibited a fragmented sleep architecture and impaired sleep homeostasis responses as revealed by the increased NREM sleep latencies, decreased slow-wave activities and spindle rate after sleep deprivation. These changes were associated with altered bursting activity and firing dynamics of neurons from the thalamic reticularis nucleus, anterior cingulate and anterodorsal thalamus. Administration of N-acetylcysteine (NAC), a clinically relevant antioxidant, rescued the sleep fragmentation and spindle rate through a renormalization of local neuronal dynamics in Gclm KO mice. Collectively, these findings provide novel evidence for a link between OxS and the deficits of frontal TC network dynamics as a possible mechanism underlying sleep abnormalities and impaired homeostatic responses observed in schizophrenia.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Sahli Building > Forschungsgruppe Neurologie
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology

UniBE Contributor:

Czekus, Christina, Orero Lopez, Albert, Bozic, Ivan, Rusterholz, Thomas, Bandarabadi, Mojtaba, Gutierrez Herrera, Carolina

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1476-5578

Publisher:

Springer Nature

Language:

English

Submitter:

Pubmed Import

Date Deposited:

02 Aug 2022 09:28

Last Modified:

11 Dec 2022 00:12

Publisher DOI:

10.1038/s41380-022-01700-w

PubMed ID:

35902628

BORIS DOI:

10.48350/171656

URI:

https://boris.unibe.ch/id/eprint/171656

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