Preparation of hybrid samples for scanning electron microscopy (SEM) coupled to focused ion beam (FIB) analysis: A new way to study cell adhesion to titanium implant surfaces.

Parisi, Ludovica; Toffoli, Andrea; Ghezzi, Benedetta; Lagonegro, Paola; Trevisi, Giovanna; Macaluso, Guido M (2022). Preparation of hybrid samples for scanning electron microscopy (SEM) coupled to focused ion beam (FIB) analysis: A new way to study cell adhesion to titanium implant surfaces. PLoS ONE, 17(8), e0272486. Public Library of Science 10.1371/journal.pone.0272486

[img]
Preview
Text
journal.pone.0272486.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (671kB) | Preview

The study of the intimate connection occurring at the interface between cells and titanium implant surfaces is a major challenge for dental materials scientists. Indeed, several imaging techniques have been developed and optimized in the last decades, but an optimal method has not been described yet. The combination of the scanning electron microscopy (SEM) with a focused ion beam (FIB), represents a pioneering and interesting tool to allow the investigation of the relationship occurring at the interface between cells and biomaterials, including titanium. However, major caveats concerning the nature of the biological structures, which are not conductive materials, and the physico-chemical properties of titanium (i.e. color, surface topography), require a fine and accurate preparation of the sample before its imaging. Hence, the aim of the present work is to provide a suitable protocol for cell-titanium sample preparation before imaging by SEM-FIB. The concepts presented in this paper are also transferrable to other fields of biomaterials research.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics

UniBE Contributor:

Parisi, Ludovica

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Pubmed Import

Date Deposited:

03 Aug 2022 11:57

Last Modified:

05 Dec 2022 16:22

Publisher DOI:

10.1371/journal.pone.0272486

PubMed ID:

35917303

BORIS DOI:

10.48350/171707

URI:

https://boris.unibe.ch/id/eprint/171707

Actions (login required)

Edit item Edit item
Provide Feedback