Further spread of perch rhabdovirus on European percid farms.

Pallandre, Laurane; Schmidt-Posthaus, Heike; Pozet, Françoise; Rupp, Mélanie; Cristina, Elodie; Bigarré, Laurent (2022). Further spread of perch rhabdovirus on European percid farms. Journal of fish diseases, 45(12), pp. 1831-1837. Wiley 10.1111/jfd.13705

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Variants of perch rhabdovirus (PRV) circulate across European percid farms via the fish trade. To trace their circulation, they are usually isolated by cell culture and subsequently identified genetically by sequencing partial or complete genes. Here, a newly developed nested PCR-based method was used to amplify and sequence the complete N and P genes directly from clinical samples obtained during an outbreak on a farm as well as from four batches of fish sampled from two other farms in another country. In an attempt to trace the origin of the five detected viruses, their N and P sequences were concatenated and compared with related viruses. One virus found in pike-perch was highly related to a virus isolated in 2016 in Belgium. Two other viruses detected on a single farm were distinct from one another, with one being almost identical to another virus isolated in 2016 in Belgium and the other being more closely related to a subgroup with different origins, France and Belgium. Two other viruses found in perch from a third farm were identical and were more related to a subgroup of viruses isolated in France. Identifying variants by a direct PCR approach will help to prevent further dissemination in farms.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Schmidt-Posthaus, Heike, Cristina, Elodie

Subjects:

600 Technology > 630 Agriculture

ISSN:

1365-2761

Publisher:

Wiley

Language:

English

Submitter:

Pubmed Import

Date Deposited:

17 Aug 2022 14:31

Last Modified:

05 Dec 2022 16:22

Publisher DOI:

10.1111/jfd.13705

PubMed ID:

35962585

Uncontrolled Keywords:

Perhabdovirus Rhabdoviridae aquaculture fish percid

BORIS DOI:

10.48350/171980

URI:

https://boris.unibe.ch/id/eprint/171980

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