A fluorescently labeled dendronized polymer-enzyme conjugate carrying multiple copies of two different types of active enzymes

Grotzky, Andrea; Nauser, Thomas; Erdogan, Huriye; Schlüter, A Dieter; Walde, Peter (2012). A fluorescently labeled dendronized polymer-enzyme conjugate carrying multiple copies of two different types of active enzymes. Journal of the American Chemical Society, 134(28), pp. 11392-5. Washington, D.C.: American Chemical Society 10.1021/ja304837f

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A hybrid structure of a synthetic dendronized polymer, two different types of enzymes (superoxide dismutase and horseradish peroxidase), and a fluorescent dye (fluorescein) was synthesized. Thereby, a single polymer chain carried multiple copies of the two enzymes and the fluorescein. The entire attachment chemistry is based on UV/vis-quantifiable bis-aryl hydrazone bond formation that allows direct quantification of bound molecules: 60 superoxide dismutase, 120 horseradish peroxidase, and 20 fluorescein molecules on an average polymer chain of 2000 repeating units. To obtain other enzyme ratios the experimental conditions were altered accordingly. Moreover, it could be shown that both enzymes remained fully active and catalyzed a two-step cascade reaction.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Clinic of Nuclear Medicine
UniBE Contributor:	Grotzky, Andrea
ISSN:	0002-7863
Publisher:	American Chemical Society
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:42
Last Modified:	05 Dec 2022 14:13
Publisher DOI:	10.1021/ja304837f
PubMed ID:	22741539
Web of Science ID:	000306457900025
URI:	https://boris.unibe.ch/id/eprint/17212 (FactScience: 224954)