Villabruna, Nele; Izquierdo-Lara, Ray W; Schapendonk, Claudia M E; de Bruin, Erwin; Chandler, Felicity; Thi Nhu Thao, Tran; Westerhuis, Brenda M; van Beek, Janko; Sigfrid, Louise; Giaquinto, Carlo; Goossens, Herman; Bielicki, Julia A; Kohns Vasconcelos, Malte; Fraaij, Pieter L A; Koopmans, Marion P G; de Graaf, Miranda (2022). Profiling of humoral immune responses to norovirus in children across Europe. Scientific Reports, 12(1), p. 14275. Nature Publishing Group 10.1038/s41598-022-18383-6
|
Text
s41598-022-18383-6.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (3MB) | Preview |
Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Tran, Thi Nhu Thao |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2045-2322 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
24 Aug 2022 10:23 |
Last Modified: |
05 Dec 2022 16:23 |
Publisher DOI: |
10.1038/s41598-022-18383-6 |
PubMed ID: |
35995986 |
BORIS DOI: |
10.48350/172339 |
URI: |
https://boris.unibe.ch/id/eprint/172339 |
Actions (login required)
 |
Edit item |