Wang, Chundi; Solberg, Therese; Maurer-Alcalá, Xyrus X; Swart, Estienne C; Gao, Feng; Nowacki, Mariusz (2022). A small RNA-guided PRC2 complex eliminates DNA as an extreme form of transposon silencing. Cell reports, 40(8), p. 111263. Cell Press 10.1016/j.celrep.2022.111263
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In animal germlines, transposons are silenced at the transcriptional or post-transcriptional level to prevent deleterious expression. Ciliates employ a more direct approach by physically eliminating transposons from their soma, utilizing piRNAs to recognize transposons and imprecisely excise them. Ancient, mutated transposons often do not require piRNAs and are precisely eliminated. Here, we characterize the Polycomb Repressive Complex 2 (PRC2) in Paramecium and demonstrate its involvement in the removal of transposons and transposon-derived DNA. Our results reveal a striking difference between the elimination of new and ancient transposons at the chromatin level and show that the complex may be guided by Piwi-bound small RNAs (sRNAs). We propose that imprecise elimination in ciliates originates from an ancient transposon silencing mechanism, much like in plants and metazoans, through sRNAs, repressive methylation marks, and heterochromatin formation. However, it is taken a step further by eliminating DNA as an extreme form of transposon silencing.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Wang, Chundi, Solberg, Therese, Maurer-Alcalá, Xyrus Xavier, Nowacki, Mariusz |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2211-1247 |
Publisher: |
Cell Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
29 Aug 2022 16:45 |
Last Modified: |
05 Dec 2022 16:23 |
Publisher DOI: |
10.1016/j.celrep.2022.111263 |
PubMed ID: |
36001962 |
Uncontrolled Keywords: |
CP: Molecular biology DNA elimination H3K27me3 H3K9me3 PRC Piwi ciliates heterochromatin piRNA transposable elements transposons |
BORIS DOI: |
10.48350/172356 |
URI: |
https://boris.unibe.ch/id/eprint/172356 |
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