CASC11 and PVT1 spliced transcripts play an oncogenic role in colorectal carcinogenesis.

Zamani, Mina; Foroughmand, Ali-Mohammad; Hajjari, Mohammad-Reza; Bakhshinejad, Babak; Johnson, Rory; Galehdari, Hamid (2022). CASC11 and PVT1 spliced transcripts play an oncogenic role in colorectal carcinogenesis. Frontiers in oncology, 12(954634), p. 954634. Frontiers Research Foundation 10.3389/fonc.2022.954634

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Cancer is fundamentally a genetic disorder that alters cellular information flow toward aberrant growth. The coding part accounts for less than 2% of the human genome, and it has become apparent that aberrations within the noncoding genome drive important cancer phenotypes. The numerous carcinogenesis-related genomic variations in the 8q24 region include single nucleotide variations (SNVs), copy number variations (CNVs), and viral integrations occur in the neighboring areas of the MYC locus. It seems that MYC is not the only target of these alterations. The MYC-proximal mutations may act via regulatory noncoding RNAs (ncRNAs). In this study, gene expression analyses indicated that the expression of some PVT1 spliced linear transcripts, CircPVT1, CASC11, and MYC is increased in colorectal cancer (CRC). Moreover, the expression of these genes is associated with some clinicopathological characteristics of CRC. Also, in vitro studies in CRC cell lines demonstrated that CASC11 is mostly detected in the nucleus, and different transcripts of PVT1 have different preferences for nuclear and cytoplasmic parts. Furthermore, perturbation of PVT1 expression and concomitant perturbation in PVT1 and CASC11 expression caused MYC overexpression. It seems that transcription of MYC is under regulatory control at the transcriptional level, i.e., initiation and elongation of transcription by its neighboring genes. Altogether, the current data provide evidence for the notion that these noncoding transcripts can significantly participate in the MYC regulation network and in the carcinogenesis of colorectal cells.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Johnson, Rory Baldwin


600 Technology > 610 Medicine & health




Frontiers Research Foundation




Pubmed Import

Date Deposited:

05 Sep 2022 15:26

Last Modified:

05 Dec 2022 16:23

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

8q24 CASC11 MYC PVT1 colorectal cancer noncoding RNA




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