Determination of voriconazole in human serum and plasma by micellar electrokinetic chromatography

Theurillat, Regula; Zimmerli, Stefan; Thormann, Wolfgang (2010). Determination of voriconazole in human serum and plasma by micellar electrokinetic chromatography. Journal of pharmaceutical and biomedical analysis, 53(5), pp. 1313-8. Oxford: Pergamon Press 10.1016/j.jpba.2010.05.015

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The micellar electrokinetic capillary chromatography (MEKC) separation and analysis of voriconazole and UK 115794 (internal standard) were examined and an assay for determination of voriconazole in human plasma and serum was developed. The MEKC medium comprises a 2:15 (v/v) mixture of methanol and a pH 9.3 buffer composed of 5mM Na(2)B(4)O(7), 7 mM Na(2)HPO(4) and 54 mM SDS. Sample preparation is based upon liquid/liquid extraction with ethylacetate and dichloromethane (75%/25%) at physiological pH. Using this approach with 250 microl serum or plasma and reconstitution of the dried extract into 100 microl of a buffer composed of 0.5mM Na(2)B(4)O(7) and 0.7 mM Na(2)HPO(4) (pH 9.3), the detection and quantitation limits were determined to be 0.1 and 0.2 microg/ml, respectively, a sensitivity that is suitable for therapeutic drug monitoring of voriconazole (provisional therapeutic range: 1-6 microg/ml) in human plasma and serum samples. The method was validated and compared to an HPLC method, showing excellent agreement between the two for a set of 91 samples that stemmed from patients being treated with voriconazole. The MEKC assay is also demonstrated to be suitable to explore pharmacokinetic data of voriconazole.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued]

UniBE Contributor:

Zimmerli, Stefan and Thormann, Wolfgang

ISSN:

0731-7085

Publisher:

Pergamon Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:11

Last Modified:

06 Dec 2013 13:21

Publisher DOI:

10.1016/j.jpba.2010.05.015

PubMed ID:

20541340

Web of Science ID:

000281576500026

URI:

https://boris.unibe.ch/id/eprint/1728 (FactScience: 203668)

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