Fluorescence Video Angiography for Evaluation of Dynamic Perfusion Status in an Aneurysm Preclinical Experimental Setting.

Grüter, Basil Erwin; Täschler, Dominik; Rey, Jeannine; Strange, Fabio; Nevzati, Edin; Fandino, Javier; Marbacher, Serge; Coluccia, Daniel (2019). Fluorescence Video Angiography for Evaluation of Dynamic Perfusion Status in an Aneurysm Preclinical Experimental Setting. Operative neurosurgery, 17(4), pp. 432-438. Oxford University Press 10.1093/ons/opz011

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BACKGROUND

Experimental studies to assess aneurysm occlusion or perfusion typically rely on macroscopic examination or histological analysis but cannot assess dynamic perfusion.

OBJECTIVE

To describe an easy-to-implement and inexpensive fluorescence angiographic technique for the in vivo assessment and imaging of the dynamic perfusion status of aneurysms and their underlying blood vessels in a rat model.

METHODS

In a rat sidewall aneurysm model, the angiographic setup included 2 bandpass filters, a video camera, and a bicycle spotlight. After 48 rats underwent fluorescein angiography, dissections were performed to confirm the perfusion status by macroscopic and histologic examination of the aneurysm.

RESULTS

Direct injection of 0.2 mL fluorescein 10% Faure achieved strong, clear visibility in all 48 aneurysms. Macro-/microscopic examination identified residual perfusion in 25 and complete healing in 23 aneurysms. Fluorescein imaging identified 21 of these 25 aneurysms (84%) with residual perfusion and 22 of 23 aneurysms (96%) with no residual perfusion.

CONCLUSION

Our fluorescein imaging technique proved efficient for the evaluation of aneurysm patency and parent artery integrity in this experimental setting. Fluorescein is nontoxic, can be re-administered if needed, and, in this technique, can expand the armamentarium for the preclinical evaluation of dynamic perfusion status.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Neurochirurgie

UniBE Contributor:

Fandino, Javier and Marbacher, Serge

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2332-4260

Publisher:

Oxford University Press

Language:

English

Submitter:

Marceline Brodmann

Date Deposited:

15 Sep 2022 14:50

Last Modified:

05 Dec 2022 16:24

Publisher DOI:

10.1093/ons/opz011

PubMed ID:

30851036

Uncontrolled Keywords:

Aneurysm Experimental Fluorescein Fluorescence Rat Video angiography

BORIS DOI:

10.48350/172912

URI:

https://boris.unibe.ch/id/eprint/172912

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