Stähli, Alexandra; Scherler, Carina; Zappalà, Graziano; Sculean, Anton; Eick, Sigrun (2022). In vitro activity of anti-rheumatic drugs on release of pro-inflammatory cytokines from oral cells in interaction with microorganisms. Frontiers in oral health, 3, p. 960732. Frontiers Media 10.3389/froh.2022.960732
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Periodontitis patients suffering concomitantly from rheumatoid arthritis (RA) often present with less inflamed periodontal tissues due to the ongoing anti-rheumatic therapy. This in vitro study was aimed to analyze whether anti-inflammatory drugs used in the therapy of RA can modulate the release of IL-8 and IL-1β by professional and non-professional immune cells stimulated with microorganisms. Periodontal ligament (PDL) fibroblasts, monocytic MONO-MAC-6-cells, and gingival keratinocytes were exposed to ibuprofen, prednisolone, and methotrexate with and without lysates of Fusobacterium nucleatum or Candida albicans. Supernatants were obtained and the levels of interleukin(IL)-8 and IL-1β (only MONO-MAC-6) were quantified. The addition of F. nucleatum lysate resulted in the strongest release of proinflammatory cytokines by PDL fibroblast and MONO-MAC-6 cells, while the modification by the tested anti-rheumatic drugs was only minor. After stimulation of the MONO-MAC-cells with F. nucleatum, prednisolone increased the release of IL-8, whereas methotrexate decreased the level. Anti-inflammatory drugs increased the adherence of C. albicans to epithelial cells. In patients with RA, the reduction of the microbial load in subgingival biofilm (biofilm removal) is of major importance; however, the intake of inflammatory drugs may interfere with the inflammatory response.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Periodontics Research 04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology |
UniBE Contributor: |
Stähli, Alexandra Beatrice, Sculean, Anton, Eick, Sigrun |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2673-4842 |
Publisher: |
Frontiers Media |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
21 Sep 2022 07:41 |
Last Modified: |
05 Dec 2022 16:24 |
Publisher DOI: |
10.3389/froh.2022.960732 |
PubMed ID: |
36118051 |
Uncontrolled Keywords: |
anti-inflammatory drugs oral cells periodontitis proinflammatory cytokines rheumatoid arthritis |
BORIS DOI: |
10.48350/173092 |
URI: |
https://boris.unibe.ch/id/eprint/173092 |
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