DNA-PKcs promotes fork reversal and chemoresistance.

Dibitetto, Diego; Marshall, Shannon; Sanchi, Andrea; Liptay, Martin; Badar, Jumana; Lopes, Massimo; Rottenberg, Sven; Smolka, Marcus B (2022). DNA-PKcs promotes fork reversal and chemoresistance. Molecular cell, 82(20), 3932-3942.e6. Cell Press 10.1016/j.molcel.2022.08.028

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The DNA-PKcs kinase mediates the repair of DNA double-strand breaks via classical non-homologous end joining (NHEJ). DNA-PKcs is also recruited to active replication forks, although a role for DNA-PKcs in the control of fork dynamics is unclear. Here, we identify a crucial role for DNA-PKcs in promoting fork reversal, a process that stabilizes stressed replication forks and protects genome integrity. DNA-PKcs promotes fork reversal and slowing in response to several replication stress-inducing agents in a manner independent of its role in NHEJ. Cells lacking DNA-PKcs activity show increased DNA damage during S-phase and cellular sensitivity to replication stress. Notably, prevention of fork slowing and reversal via DNA-PKcs inhibition efficiently restores chemotherapy sensitivity in BRCA2-deficient mammary tumors with acquired PARPi resistance. Together, our data uncover a new key regulator of fork reversal and show how DNA-PKcs signaling can be manipulated to alter fork dynamics and drug resistance in cancer.

Item Type:

Journal Article (Original Article)


05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

UniBE Contributor:

Dibitetto, Diego, Liptay, Martin, Rottenberg, Sven


600 Technology > 630 Agriculture




Cell Press




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Date Deposited:

23 Sep 2022 11:03

Last Modified:

05 Dec 2022 16:25

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Uncontrolled Keywords:

BRCA1 BRCA2 DNA replication DNA-PKcs NHEJ PARP inhibitor chemoresistance fork reversal





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