Computer-Aided Histopathological Characterisation of Endometriosis Lesions.

Mc Kinnon, Brett D; Nirgianakis, Konstantinos; Ma, Lijuan; Wotzkow Alvarez, Carlos; Steiner, Selina; Blank, Fabian; Mueller, Michael D (2022). Computer-Aided Histopathological Characterisation of Endometriosis Lesions. Journal of personalized medicine, 12(9) MDPI 10.3390/jpm12091519

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Endometriosis is a common gynaecological condition characterised by the growth of endometrial tissue outside the uterus and is associated with pain and infertility. Currently, the gold standard for endometriosis diagnosis is laparoscopic excision and histological identification of endometrial epithelial and stromal cells. There is, however, currently no known association between the histological appearance, size, morphology, or subtype of endometriosis and disease prognosis. In this study, we used histopathological software to identify and quantify the number of endometrial epithelial and stromal cells within excised endometriotic lesions and assess the relationship between the cell contents and lesion subtypes. Prior to surgery for suspected endometriosis, patients provided menstrual and abdominal pain and dyspareunia scores. Endometriotic lesions removed during laparoscopic surgery were collected and prepared for immunohistochemistry from 26 patients. Endometrial epithelial and stromal cells were identified with Cytokeratin and CD10 antibodies, respectively. Whole slide sections were digitised and the QuPath software was trained to automatically detect and count epithelial and stromal cells across the whole section. Using this classifier, we identified a significantly larger number of strongly labelled CD10 stromal cells (p = 0.0477) in deeply infiltrating lesions (99,970 ± 2962) compared to superficial lesions (2456 ± 859). We found the ratio of epithelial to stromal cells was inverted in deeply infiltrating endometriosis lesions compared to superficial peritoneal and endometrioma lesions and we subsequently identified a correlation between total endometrial cells and abdominal pain (p = 0.0005) when counted via the automated software. Incorporating histological software into current standard diagnostic pipelines may improve endometriosis diagnosis and provide prognostic information in regards to severity and symptoms and eventually provide the potential to personalise adjuvant treatment decisions.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Live Cell Imaging (LCI)
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

UniBE Contributor:

Mc Kinnon, Brett, Nirgianakis, Konstantinos, Ma, Lijuan, Wotzkow Alvarez, Carlos, Steiner, Selina Katja, Blank, Fabian, Mueller, Michael


600 Technology > 610 Medicine & health








Pubmed Import

Date Deposited:

26 Sep 2022 13:58

Last Modified:

05 Dec 2022 16:25

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PubMed ID:


Uncontrolled Keywords:

CD10 Qupath cytokeratin endometriosis epithelial histopathology pain stromal subtype




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