Assay of Endocannabinoid Uptake.

Reynoso-Moreno, Ines; Rau, Mark; Chicca, Andrea; Nicolussi, Simon; Gertsch, Jürg (2023). Assay of Endocannabinoid Uptake. Methods in molecular biology, 2576, pp. 329-348. Springer 10.1007/978-1-0716-2728-0_28

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Endocannabinoids at nanomolar physiological concentrations cross cellular membranes by facilitated diffusion, a process that can be studied by measuring transport kinetics and endocannabinoid trafficking employing radioligands and mass spectrometry. Here, we describe radiosubstrate-based assays using arachidonoyl[1-3H]ethanolamine and 2-arachidonoyl[1,2,3-3H]glycerol to measure cellular endocannabinoid uptake in a three-phase assay with human U937 cells. Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS)-based lipidomics was used to interrogate the roles of serum and albumin for endocannabinoid trafficking in U937 cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Reynoso, Ines del Carmen, Rau, Mark, Chicca, Andrea, Nicolussi, Simon, Gertsch, Jürg

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1940-6029

Publisher:

Springer

Language:

English

Submitter:

Pubmed Import

Date Deposited:

26 Sep 2022 13:48

Last Modified:

05 Dec 2022 16:25

Publisher DOI:

10.1007/978-1-0716-2728-0_28

PubMed ID:

36152200

Uncontrolled Keywords:

2-Arachidonoylglycerol Anandamide Endocannabinoid uptake LC–ESI–MS/MS

BORIS DOI:

10.48350/173258

URI:

https://boris.unibe.ch/id/eprint/173258

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