Detecting aquatic pathogens with field-compatible dried qPCR assays.

Rieder, Jessica; Martin-Sanchez, Pedro M; Osman, Omneya A; Adrian-Kalchhauser, Irene; Eiler, Alexander (2022). Detecting aquatic pathogens with field-compatible dried qPCR assays. Journal of microbiological methods, 202(106594), p. 106594. Elsevier 10.1016/j.mimet.2022.106594

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Field-ready qPCR assays with a long shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf-life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Rieder, Jessica Marie, Adrian-Kalchhauser, Irene

Subjects:

600 Technology > 630 Agriculture

ISSN:

0167-7012

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

17 Oct 2022 12:13

Last Modified:

13 Oct 2023 00:25

Publisher DOI:

10.1016/j.mimet.2022.106594

PubMed ID:

36241007

Uncontrolled Keywords:

Air-dried assay Aquatic pathology Environmental diagnostics Lyophilization eDNA qPCR

BORIS DOI:

10.48350/173760

URI:

https://boris.unibe.ch/id/eprint/173760

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