Plasticity of the adult human small intestinal stoma microbiota.

Yilmaz, Bahtiyar; Fuhrer, Tobias; Morgenthaler, Deborah; Krupka, Niklas; Wang, Daoming; Spari, Daniel; Candinas, Daniel; Misselwitz, Benjamin; Beldi, Guido; Sauer, Uwe; Macpherson, Andrew J (2022). Plasticity of the adult human small intestinal stoma microbiota. Cell host & microbe, 30(12), 1773-1787.e6. Cell Press 10.1016/j.chom.2022.10.002

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The human distal small intestine (ileum) has a distinct microbiota, but human studies investigating its composition and function have been limited by the inaccessibility of the ileum without purging and/or deep intubation. We investigated inherent instability, temporal dynamics, and the contribution of fed and fasted states using stoma samples from cured colorectal cancer patients as a non-invasive access route to the otherwise inaccessible small and large intestines. Sequential sampling of the ileum before and after stoma formation indicated that ileostoma microbiotas represented that of the intact small intestine. Ileal and colonic stoma microbiotas were confirmed as distinct, and two types of instability in ileal host-microbial relationships were observed: inter-digestive purging followed by the rapid postprandial blooming of bacterial biomass and sub-strain appearance and disappearance within individual taxa after feeding. In contrast to the relative stability of colonic microbiota, the human small intestinal microbiota biomass and its sub-strain composition can be highly dynamic.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Gastroenterologie / Mukosale Immunologie

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Yilmaz, Bahtiyar (A), Morgenthaler, Deborah, Krupka, Niklas, Spari, Daniel, Candinas, Daniel, Misselwitz, Benjamin, Beldi, Guido Jakob Friedrich, Macpherson, Andrew

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1931-3128

Publisher:

Cell Press

Language:

English

Submitter:

Pubmed Import

Date Deposited:

02 Nov 2022 10:08

Last Modified:

17 Dec 2022 00:14

Publisher DOI:

10.1016/j.chom.2022.10.002

PubMed ID:

36318918

Uncontrolled Keywords:

bacterial biomass colostomy ileostomy metabolomics microbial instability small intestinal microbiota sub-strains

BORIS DOI:

10.48350/174411

URI:

https://boris.unibe.ch/id/eprint/174411

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