Kaplan, Lew; Drexler, Corinne; Pfaller, Anna M; Brenna, Santra; Wunderlich, Kirsten A; Dimitracopoulos, Andrea; Merl-Pham, Juliane; Perez, Maria-Theresa; Schlötzer-Schrehardt, Ursula; Enzmann, Volker; Samardzija, Marijana; Puig, Berta; Fuchs, Peter; Franze, Kristian; Hauck, Stefanie M; Grosche, Antje (2023). Retinal regions shape human and murine Müller cell proteome profile and functionality. GLIA, 71(2), pp. 391-414. Wiley-Blackwell 10.1002/glia.24283
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Glia_-_2022_-_Kaplan_-_Retinal_regions_shape_human_and_murine_M_ller_cell_proteome_profile_and_functionality.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (5MB) | Preview |
The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde |
UniBE Contributor: |
Enzmann, Volker |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0894-1491 |
Publisher: |
Wiley-Blackwell |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
07 Nov 2022 12:24 |
Last Modified: |
16 Dec 2022 00:14 |
Publisher DOI: |
10.1002/glia.24283 |
PubMed ID: |
36334068 |
Uncontrolled Keywords: |
EPPK1 Müller cells glial heterogeneity macula retina |
BORIS DOI: |
10.48350/174532 |
URI: |
https://boris.unibe.ch/id/eprint/174532 |
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