Development of an LC-MS/MS method for the quantitation of 55 compounds prescribed in combined cardiovascular therapy

Gonzalez, Oskar; Alonso, Rosa Maria; Ferreirós, Nerea; Weinmann, Wolfgang; Zimmermann, Ralf; Dresen, Sebastian (2011). Development of an LC-MS/MS method for the quantitation of 55 compounds prescribed in combined cardiovascular therapy. Journal of chromatography. B - analytical technologies in the biomedical and life sciences, 879(3-4), pp. 243-52. New York, N.Y.: Elsevier 10.1016/j.jchromb.2010.12.007

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This paper reports an LC-MS/MS method with positive electrospray ionization for the screening of commonly prescribed cardiovascular drugs in human plasma, including compounds with antihypertensive (57), antidiabetic (12), hypolipemiant (5), anticoagulant (2) and platelet anti-aggregation (2) effects. Sample treatment consisted of a simple protein precipitation with MeOH/0.1 M ZnSO₄ (4:1, v/v) solution after the addition of internal standard, followed by evaporation and reconstitution. Analytes separation was performed on a Polar-RP column (150 m x 2 mm, 4 μm) using a gradient elution of 15 min. The MS system was operated in MRM mode, monitoring one quantitation and one confirmation transition for each analyte. The recovery of the protein precipitation step ranged from 50 to 70% for most of the compounds, while some were considerably affected by matrix effects. Since several analytes fulfilled the linearity, accuracy and precision values required by the ICH guidelines, the method proved to be suitable for their quantitative analysis. The limits of quantitation varied from 0.38 to 9.1 μg/L and the limits of detection from 0.12 to 5.34 μg/L. The method showed to be suitable for the detection of plasma samples of patients under cardiovascular treatment with the studied drugs, and for 55 compounds reliable quantitative results could be obtained.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Chemistry and Toxicology

UniBE Contributor:

Weinmann, Wolfgang

ISSN:

1570-0232

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:11

Last Modified:

17 Mar 2015 19:12

Publisher DOI:

10.1016/j.jchromb.2010.12.007

PubMed ID:

21190906

Web of Science ID:

000287269800003

URI:

https://boris.unibe.ch/id/eprint/1746 (FactScience: 203697)

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