Leni, Zaira; Ess, Michaela N; Keller, Alejandro; Allan, James D; Hellén, Heidi; Saarnio, Karri; Williams, Katie R; Brown, Andrew S; Salathe, Matthias; Baumlin, Nathalie; Vasilatou, Konstantina; Geiser, Marianne (2022). Role of Secondary Organic Matter on Soot Particle Toxicity in Reconstituted Human Bronchial Epithelia Exposed at the Air-Liquid Interface. Environmental science & technology, 56(23), pp. 17007-17017. ACS Publications 10.1021/acs.est.2c03692
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Secondary organic matter (SOM) formed from gaseous precursors constitutes a major mass fraction of fine particulate matter. However, there is only limited evidence on its toxicological impact. In this study, air-liquid interface cultures of human bronchial epithelia were exposed to different series of fresh and aged soot particles generated by a miniCAST burner combined with a micro smog chamber (MSC). Soot cores with geometric mean mobility diameters of 30 and 90 nm were coated with increasing amounts of SOM, generated from the photo-oxidation of mesitylene and ozonolysis of α-pinene. At 24 h after exposure, the release of lactate dehydrogenase (LDH), indicating cell membrane damage, was measured and proteome analysis, i.e. the release of 102 cytokines and chemokines to assess the inflammatory response, was performed. The data indicate that the presence of the SOM coating and its bioavailability play an important role in cytotoxicity. In particular, LDH release increased with increasing SOM mass/total particle mass ratio, but only when SOM had condensed on the outer surface of the soot cores. Proteome analysis provided further evidence for substantial interference of coated particles with essential properties of the respiratory epithelium as a barrier as well as affecting cell remodeling and inflammatory activity.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology |
UniBE Contributor: |
Leni, Zaira, Geiser, Marianne |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
0013-936X |
Publisher: |
ACS Publications |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
24 Nov 2022 09:55 |
Last Modified: |
07 Dec 2022 00:16 |
Publisher DOI: |
10.1021/acs.est.2c03692 |
PubMed ID: |
36416368 |
Uncontrolled Keywords: |
aerosol air−liquid interface exposure bronchial epithelial cells cytotoxicity oxidation flow reactor pro-inflammatory cytokines secondary organic matter soot |
BORIS DOI: |
10.48350/175111 |
URI: |
https://boris.unibe.ch/id/eprint/175111 |
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