Systemic Lipopolysaccharide Exposure Exacerbates Choroidal Neovascularization in Mice.

Tsioti, Ioanna; Steiner, Beatrice L; Escher, Pascal; Zinkernagel, Martin S; Benz, Peter M; Kokona, Despina (2024). Systemic Lipopolysaccharide Exposure Exacerbates Choroidal Neovascularization in Mice. Ocular immunology and inflammation, 32(1), pp. 19-30. Taylor & Francis 10.1080/09273948.2022.2147547

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This study aims to investigate the effect of a systemic lipopolysaccharide (LPS) stimulus in the course of laser-induced choroidal neovascularization (CNV) in C57BL/6 J mice. A group of CNV-subjected mice received 1 mg/kg LPS via the tail vein immediately after CNV induction. Mouse eyes were monitored in vivo with fluorescein angiography for 2 weeks. In situ hybridization and flow cytometry were performed in the retina at different time points. LPS led to increased fluorescein leakage 3 days after CNV, correlated with a large influx of monocyte-derived macrophages and increase of pro-inflammatory microglia/macrophages in the retina. Additionally, LPS enhanced Vegfα mRNA expression by Glul-expressing cells but not Aif1 positive microglia/macrophages in the laser lesion. These findings suggest that systemic LPS exposure has transient detrimental effects in the course of CNV through activation of microglia/macrophages to a pro-inflammatory phenotype and supports the important role of these cells in the CNV course.

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