Gnann, H; Engelmann, C; Skopp, G; Winkler, M; Auwärter, V; Dresen, S; Ferreirós, N; Wurst, F M; Weinmann, W (2010). Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS/MS. Analytical and bioanalytical chemistry, 396(7), pp. 2415-23. Heidelberg: Springer 10.1007/s00216-010-3458-5
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Phosphatidylethanol (PEth) is an abnormal phospholipid carrying two fatty acid chains. It is only formed in the presence of ethanol via the action of phospholipase D (PLD). Its use as a biomarker for alcohol consumption is currently under investigation. Previous methods for the analysis of PEth included high-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD), which is unspecific for the different homologues--improved methods are now based on time of flight mass spectrometry (TOF-MS) and tandem mass spectrometry (MS/MS). The intention of this work was to identify as many homologues of PEth as possible. A screening procedure using multiple-reaction monitoring (MRM) for the identified homologues has subsequently been established. For our investigations, autopsy blood samples collected from heavy drinkers were used. Phosphatidylpropanol 16:0/18:1 (internal standard) was added to the blood samples prior to liquid-liquid extraction using borate buffer (pH 9), 2-propanol and n-hexane. After evaporation, the samples were redissolved in the mobile phase and injected into the LC-MS/MS system. Compounds were separated on a Luna Phenyl Hexyl column (50 mm x 2 mm, 3 microm) by gradient elution, using 2 mM ammonium acetate and methanol/acetone (95/5; v/v). A total of 48 homologues of PEth could be identified by using precursor ion and enhanced product ion scans (EPI).
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Chemistry and Toxicology |
UniBE Contributor: |
Weinmann, Wolfgang |
ISSN: |
1618-2642 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:11 |
Last Modified: |
05 Dec 2022 14:01 |
Publisher DOI: |
10.1007/s00216-010-3458-5 |
PubMed ID: |
20127079 |
Web of Science ID: |
000275752600005 |
BORIS DOI: |
10.7892/boris.1753 |
URI: |
https://boris.unibe.ch/id/eprint/1753 (FactScience: 203704) |
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