Jin, Yao; Adams, Friederike; Möller, Judith; Isert, Lorenz; Zimmermann, Christoph M; Keul, David; Merkel, Olivia M (2023). Synthesis and Application of Low Molecular Weight PEI-based Copolymers for siRNA Delivery with Smart Polymer Blends. Macromolecular bioscience, 23(2), e2200409. Wiley-VCH 10.1002/mabi.202200409
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Macromolecular_Bioscience_-_2022_-_Jin_-_Synthesis_and_Application_of_Low_Molecular_Weight_PEI_based_Copolymers_for_siRNA-1.pdf - Accepted Version Available under License Publisher holds Copyright. Download (1MB) | Preview |
Polyethylenimine (PEI) is a commonly used cationic polymer for small-interfering RNA (siRNA) delivery due to its high transfection efficiency at low commercial cost. However, high molecular weight PEI is cytotoxic and thus, its practical application is limited. In this study, we investigated different formulations of low molecular weight PEI (LMW-PEI) based copolymers PEI-PCL (800 Da-40 kDa) and PEI-PCL-PEI (5 kDa-5 kDa-5 kDa) blended with or without PEG-PCL (5 kDa-4 kDa) to prepare nanoparticles via nanoprecipitation using a solvent displacement method with sizes around 100 nm. PEG-PCL can stabilize the nanoparticles, improve their biocompatibility, and extend their circulation time in vivo. The nanoparticles composed of PEI-PCL-PEI and PEG-PCL showed higher siRNA encapsulation efficiency than PEI-PCL/PEG-PCL based nanoparticles at low N/P ratios, higher cellular uptake, and a gene silencing efficiency of around 40% as a result of the higher molecular weight PEI blocks. These results suggested that the PEI-PCL-PEI/PEG-PCL nanoparticle system could be a promising vehicle for siRNA delivery at minimal synthetic effort. This article is protected by copyright. All rights reserved.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Zimmermann, Christoph Martin |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1616-5187 |
Publisher: |
Wiley-VCH |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
30 Nov 2022 10:00 |
Last Modified: |
30 Nov 2023 00:25 |
Publisher DOI: |
10.1002/mabi.202200409 |
PubMed ID: |
36446588 |
Uncontrolled Keywords: |
PEG-PCL low molecular weight PEI nanoprecipitation siRNA delivery |
BORIS DOI: |
10.48350/175337 |
URI: |
https://boris.unibe.ch/id/eprint/175337 |
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