Tef Biotechnology

Gebre, Endale; Tibebu, Redeat; Gugsa, Likyelesh; Assefa, Kebebew; Chanyalew, Solomon; Tadele, Zerihun (2022). Tef Biotechnology. In: Assefa, Kebebew; Chanyalew, Solomon; Girma, Dejene; Tadele, Zerihun (eds.) Principles and Practices of Tef Improvement (pp. 127-157). Addis Ababa, Ethiopia: Agricultural Transformation Institute

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In vitro technology has been advancing as a highly valuable pre-breeding tool for achieving better results in germplasm preservation, distant hybridization and genetic transformation approaches. Furtheremore, it has been gaining a growing importance in direct applications in conventional line selection by facilitating asexual multiplication, inducing variations, undertaking site-directed mutagenesis and production of doubled haploids. Because of genetic difference, tissue specificity and culture environment (media) interactions, there are no universally applicable protocols of culture and regeneration pathways. It varies as tissues from different sources and species as well as varieties differ in their response and culture requirements. The challenges are more conspicuous with recalcitrant monocot species such as tef.

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