Dafalla, Maysaa; Orłowska, Anna; Keleş, Sinan Julian; Straková, Petra; Schlottau, Kore; Jeske, Kathrin; Hoffmann, Bernd; Wibbelt, Gudrun; Smreczak, Marcin; Müller, Thomas; Freuling, Conrad Martin; Wang, Xuejing; Rola, Jerzy; Drewes, Stephan; Fereidouni, Sasan; Heckel, Gerald; Ulrich, Rainer G (2023). Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe. Virus genes, 59(2), pp. 323-332. Nijhoff 10.1007/s11262-022-01952-2
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Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) |
UniBE Contributor: |
Wang, Xuejing, Heckel, Gerald |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
0920-8569 |
Publisher: |
Nijhoff |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
22 Dec 2022 09:02 |
Last Modified: |
20 Mar 2023 00:13 |
Publisher DOI: |
10.1007/s11262-022-01952-2 |
PubMed ID: |
36542315 |
Uncontrolled Keywords: |
Bats Europe Hantavirus Host Host specificity RT-qPCR |
BORIS DOI: |
10.48350/176332 |
URI: |
https://boris.unibe.ch/id/eprint/176332 |
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