Dejea, Hector; Schlepütz, Christian M.; Méndez-Carmona, Natalia; Arnold, Maria; Garcia-Canadilla, Patricia; Longnus, Sarah L.; Stampanoni, Marco; Bijnens, Bart; Bonnin, Anne (2022). A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle. Frontiers in cardiovascular medicine, 9 Frontiers 10.3389/fcvm.2022.1023483
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ntroduction: Cardiac architecture has been extensively investigated ex vivo using a broad spectrum of imaging techniques. Nevertheless, the heart is a dynamic system and the structural mechanisms governing the cardiac cycle can only be unveiled when investigating it as such.
Methods: This work presents the customization of an isolated, perfused heart system compatible with synchrotron-based X-ray phase contrast imaging (X-PCI).
Results: Thanks to the capabilities of the developed setup, it was possible to visualize a beating isolated, perfused rat heart for the very first time in 4D at an unprecedented 2.75 μm pixel size (10.6 μm spatial resolution), and 1 ms temporal resolution.
Discussion: The customized setup allows high-spatial resolution studies of heart architecture along the cardiac cycle and has thus the potential to serve as a tool for the characterization of the structural dynamics of the heart, including the effects of drugs and other substances able to modify the cardiac cycle.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Heart Surgery 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) |
UniBE Contributor: |
Méndez Carmona, Natalia, Arnold, Maria Regula, Henning Longnus, Sarah |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2297-055X |
Publisher: |
Frontiers |
Language: |
English |
Submitter: |
Paul Libera |
Date Deposited: |
05 Jan 2023 08:23 |
Last Modified: |
27 Feb 2024 14:28 |
Publisher DOI: |
10.3389/fcvm.2022.1023483 |
BORIS DOI: |
10.48350/176462 |
URI: |
https://boris.unibe.ch/id/eprint/176462 |
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