Rocha, Mariana S; Batista, João V C; Melo, Michelle N O; Campos, Vania E B de; Toledo, Anna Lecticia M M; Oliveira, Adriana P; Picciani, Paulo H S; Baumgartner, Stephan; Holandino, Carla (2022). Pluronic® F127 Thermoresponsive Viscum album Hydrogel: Physicochemical Features and Cellular In Vitro Evaluation. Pharmaceutics, 14(12) MDPI 10.3390/pharmaceutics14122775
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Viscum album L., popularly known as mistletoe, is well known for its anti-cancer properties, and the pharmaceutical application of hydroalcoholic dry extracts is still limited due to its low solubility in aqueous media, and physicochemical instability. The Pluronic® F127 is an amphiphilic polymer, which permits the solubilization of lipophilic and hydrophilic compounds. In this investigation, physicochemical features of hydrogel containing V. album dry extract (VADE-loaded-hydrogel) were performed by: dynamic light scattering (DLS), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and transmission electron microscopy (TEM). VADE-loaded-hydrogel presented nanometer-size micelles with volume distribution ranging from 10.58 nm to 246.7 nm, and a polydispersity index of 0.441. The sample thermal analyses (TG and DSC) showed similar decomposition curves; however, the thermal events indicated an increase in thermal stability in relation to the presence of the extract. In addition to these interesting pharmaceutical features, IC50 values of 333.40 µg/mL and >1000 µg/mL were obtained when tumor (SCC-25) and non-tumor (L929) cells were incubated with VADE-loaded-hydrogel, respectively. The optical and ultrastructural cellular analysis confirmed the tumor selectivity since the following alterations were detected only in SCC-25 cells: disorganization of plasmatic membrane; an increase of cytoplasmatic vacuole size; alteration in the cristae mitochondrial shape; and generation of amorphous cellular material. These results emphasize the promising antitumoral potential of VADE-loaded-hydrogel as an herbal drug delivery system via in vitro assays.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
?? 4913F1A0CB2C4238A97A353DEBF2BA6A ?? 04 Faculty of Medicine > Medical Education > Institute of Complementary and Integrative Medicine (IKIM) |
UniBE Contributor: |
Baumgartner, Stephan |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1999-4923 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
09 Jan 2023 12:21 |
Last Modified: |
21 Apr 2023 13:49 |
Publisher DOI: |
10.3390/pharmaceutics14122775 |
PubMed ID: |
36559269 |
Uncontrolled Keywords: |
cytotoxic assays mistletoe thermal analyses |
BORIS DOI: |
10.48350/176495 |
URI: |
https://boris.unibe.ch/id/eprint/176495 |
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