Sudworth, Amanda; Segers, Filip M; Yilmaz, Bahtiyar; Guslund, Naomi C; Macpherson, Andrew J; Dissen, Erik; Qiao, Shuo-Wang; Inngjerdingen, Marit (2022). Innate lymphoid cell characterization in the rat and their correlation to gut commensal microbes. European journal of immunology, 52(5), pp. 717-729. Wiley 10.1002/eji.202149639
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Eur_J_Immunol_-_2022_-_Sudworth_-_Innate_lymphoid_cell_characterization_in_the_rat_and_their_correlation_to_gut_commensal.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC). Download (3MB) | Preview |
Innate lymphoid cells (ILCs) are important for tissue immune homeostasis, and are thoroughly characterized in mice and humans. Here, we have performed in-depth characterization of rat ILCs. Rat ILCs were identified based on differential expression of transcription factors and lack of lineage markers. ILC3s represented the major ILC population of the small intestine, while ILC2s were infrequent but most prominent in liver and adipose tissue. Two major subsets of group 1 ILCs were defined. Lineage- T-bet+ Eomes+ cells were identified as conventional NK cells, while lineage- T-bet+ Eomes- cells were identified as the probable rat counterpart of ILC1s based on their selective expression of the ILC marker CD200R. Rat ILC1s were particularly abundant in liver and intestinal tissues, and were functionally similar to NK cells. Single-cell transcriptomics of spleen and liver cells confirmed the main division of NK cells and ILC1-like cells, and demonstrated Granzyme A as an additional ILC1 marker. We further report differential distributions of NK cells and ILCs along the small and large intestines, and the association of certain bacterial taxa to frequencies of ILCs. In conclusion, we provide a framework for future studies of ILCs in diverse rat experimental models, and novel data on the potential interplay between commensals and intestinal ILCs.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine |
UniBE Contributor: |
Yilmaz, Bahtiyar (A), Macpherson, Andrew |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1521-4141 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Rahel Fuhrer |
Date Deposited: |
09 Jan 2023 08:55 |
Last Modified: |
29 Apr 2024 23:38 |
Publisher DOI: |
10.1002/eji.202149639 |
PubMed ID: |
35099074 |
Uncontrolled Keywords: |
ILC NK cells microbiome rat |
BORIS DOI: |
10.48350/176897 |
URI: |
https://boris.unibe.ch/id/eprint/176897 |
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