Horn, Marianna E; Segner, Helmut; Brinkmann, Markus; Machtaler, Steven (2023). Chemically-induced trout model of acute intestinal inflammation using TNBS. Fish and shellfish immunology reports, 4, p. 100073. Elsevier 10.1016/j.fsirep.2022.100073
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Chemically-induced models of intestinal inflammation are a useful tool for the study of immune responses and inflammation. Although well established in mammals, application of these models is currently limited in teleosts. Based on a variety of factors, including genetic diversity, known toxicological sensitivity, and economic importance, we propose salmonids as a model family of fishes for studying intestinal inflammation. We present a rainbow trout model of chemically-induced intestinal inflammation using 2,4,6-trinitrobenzene sulfonic acid (TNBS), assessed through histological analysis of primary and secondary intestinal folding, enterocyte morphology, goblet cell size and frequency, tissue layer thickness, and immune cell infiltration. Twenty-four hours after treatment with one of three concentrations of TNBS, trout developed classic signs of intestinal inflammation, including notably increased thickness of primary and secondary folds, and increased immune cell infiltration as compared to controls. This study provides a simple, reproducible model of rapid TNBS-induction of moderate intestinal inflammation.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI) |
UniBE Contributor: |
Segner, Helmut |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2667-0119 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
10 Jan 2023 16:39 |
Last Modified: |
15 Jan 2023 02:19 |
Publisher DOI: |
10.1016/j.fsirep.2022.100073 |
PubMed ID: |
36605611 |
Uncontrolled Keywords: |
TNBS chemically-induced inflammation enteritis intestinal inflammation rainbow trout |
BORIS DOI: |
10.48350/177014 |
URI: |
https://boris.unibe.ch/id/eprint/177014 |
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