Barut, Güliz Tuba; Kreuzer, Marco; Bruggmann, Rémy; Summerfield, Artur; Talker, Stephanie C (2022). Single-cell transcriptomics reveals striking heterogeneity and functional organization of dendritic and monocytic cells in the bovine mesenteric lymph node. Frontiers in immunology, 13(1099357), p. 1099357. Frontiers Research Foundation 10.3389/fimmu.2022.1099357
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Dendritic and monocytic cells co-operate to initiate and shape adaptive immune responses in secondary lymphoid tissue. The complexity of this system is poorly understood, also because of the high phenotypic and functional plasticity of monocytic cells. We have sequenced mononuclear phagocytes in mesenteric lymph nodes (LN) of three adult cows at the single-cell level, revealing ten dendritic-cell (DC) clusters and seven monocyte/macrophage clusters with clearly distinct transcriptomic profiles. Among DC, we defined LN-resident subsets and their progenitors, as well as subsets of highly activated migratory DC differing in transcript levels for T-cell attracting chemokines. Our analyses also revealed a potential differentiation path for cDC2, resulting in a cluster of inflammatory cDC2 with close transcriptional similarity to putative DC3 and monocyte-derived DC. Monocytes and macrophages displayed sub-clustering mainly driven by pro- or anti-inflammatory expression signatures, including a small cluster of cycling, presumably self-renewing, macrophages. With this transcriptomic snapshot of LN-derived mononuclear phagocytes, we reveal functional properties and differentiation trajectories in a "command center of immunity", and identify elements that are conserved across species.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology |
UniBE Contributor: |
Barut, Güliz Tuba, Kreuzer, Marco Claudio, Bruggmann, Rémy, Summerfield, Artur, Talker, Stephanie |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1664-3224 |
Publisher: |
Frontiers Research Foundation |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
25 Jan 2023 09:20 |
Last Modified: |
05 Feb 2023 02:25 |
Publisher DOI: |
10.3389/fimmu.2022.1099357 |
PubMed ID: |
36685557 |
Uncontrolled Keywords: |
cattle dendritic cells macrophages mesenteric lymph node monocytes single-cell RNA-seq (scRNA-seq) |
BORIS DOI: |
10.48350/177809 |
URI: |
https://boris.unibe.ch/id/eprint/177809 |
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