The role of Nogo-A in neuronal differentiation in SH-SY5Y cells.

Herren, Fabienne Lois (2020). The role of Nogo-A in neuronal differentiation in SH-SY5Y cells. (Unpublished). (Dissertation, Vetsuisse)

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Parkinson's disease is one of the most common neurodegenerative disorders, but to this day the pathophysiology of the loss of dopaminergic neurons in the Substantia nigra is not fully understood. Nogo-A, one of the most potent neurite outgrowth inhibitors of the central nervous system is thought to have a neuroprotective role as well as interfering with therapeutic research, limiting the sprouting of grafted dopaminergic neurons. This study aims at further understanding the role of Nogo-A in neuronal differentiation using the neuroblastoma SH-SY5Y cells. Differentiation of SH-SY5Y cells with staurosporine into cells exhibiting a dopaminergic phenotype was not affected by antogonization of the Nogo-A cascade. SH-SY5Y cells were successfully transduced witha lentiviral vector-based system for delivery of a CRISPR/Cas9 plasmid targeting the Nogo-A gene, thus creating a Nogo-A knock-out cell line.
The creation of a Nogo-A knock-out SH-SY5Y cell population enables and facilitates further research on the involvement of Nogo-A in neuronal differentiation and degeneration.

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