Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

Rossatti, Pascal; Redpath, Gregory M I; Ziegler, Luca; Samson, Guerric P B; Clamagirand, Camille D; Legler, Daniel F; Rossy, Jérémie (2022). Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation. BMC biology, 20(1), p. 189. BioMed Central 10.1186/s12915-022-01386-0

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BACKGROUND

T cell activation leads to increased expression of the receptor for the iron transporter transferrin (TfR) to provide iron required for the cell differentiation and clonal expansion that takes place during the days after encounter with a cognate antigen. However, T cells mobilise TfR to their surface within minutes after activation, although the reason and mechanism driving this process remain unclear.

RESULTS

Here we show that T cells transiently increase endocytic uptake and recycling of TfR upon activation, thereby boosting their capacity to import iron. We demonstrate that increased TfR recycling is powered by a fast endocytic sorting pathway relying on the membrane proteins flotillins, Rab5- and Rab11a-positive endosomes. Our data further reveal that iron import is required for a non-canonical signalling pathway involving the kinases Zap70 and PAK, which controls adhesion of the integrin LFA-1 and eventually leads to conjugation with antigen-presenting cells.

CONCLUSIONS

Altogether, our data suggest that T cells boost their iron importing capacity immediately upon activation to promote adhesion to antigen-presenting cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute

UniBE Contributor:

Legler, Daniel

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1741-7007

Publisher:

BioMed Central

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

10 Feb 2023 09:00

Last Modified:

10 Feb 2023 23:27

Publisher DOI:

10.1186/s12915-022-01386-0

PubMed ID:

36002835

Uncontrolled Keywords:

Adhesion Endocytic recycling Flotillin Immunological synapse Integrins Iron Rab5/Rab11 T cell activation Transferrin Zap70

BORIS DOI:

10.48350/178603

URI:

https://boris.unibe.ch/id/eprint/178603

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