Structural properties of the extracellular biopolymer (β-D-xylo-α-D-mannan) produced by the green microalga Gloeocystis vesiculosa Nägeli.

Capek, Lukaš; Uhliariková, Iveta; Košťálová, Zuzana; Hindáková, Alica; Capek, Peter (2023). Structural properties of the extracellular biopolymer (β-D-xylo-α-D-mannan) produced by the green microalga Gloeocystis vesiculosa Nägeli. Carbohydrate research, 525(108766), p. 108766. Elsevier 10.1016/j.carres.2023.108766

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Many species of microalgae produce a relatively diverse range of metabolites that are interesting for biotechnological applications, and among them exopolysaccharides attract attention due to their structural complexity, biological activities, biodegradability or biocompatibility. An exopolysaccharide of high molecular weight (Mp) of 6.8 × 105 g/mol was obtained by cultivation of the freshwater green coccal microalga Gloeocystis vesiculosa Nägeli 1849 (Chlorophyta). Chemical analyses revealed a dominance of Manp (63.4 wt%), Xylp and its 3-O-Me-derivative (22.4 wt%), and Glcp (11.5 wt%) residues. The results of the chemical and NMR analyses showed an alternating branched 1,2- and 1,3-linked α-D-Manp backbone terminated by a single β-D-Xylp and its 3-O-methyl derivative at O2 of the 1,3-linked α-D-Manp residues. The α-D-Glcp residues were found mainly as 1,4-linked and to a lesser extent as the terminal sugar, indicating partial contamination of β-D-xylo-α-D-mannan with amylose (∼10 wt%) in G. vesiculosa exopolysaccharide.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology

UniBE Contributor:

Capek, Lukás

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1873-426X

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

23 Feb 2023 14:05

Last Modified:

18 Mar 2023 00:15

Publisher DOI:

10.1016/j.carres.2023.108766

PubMed ID:

36812847

Uncontrolled Keywords:

Extracellular β-D-xylo-α-D-mannan Gloeocystis vesiculosa Green microalgae NMR Structural properties

BORIS DOI:

10.48350/179078

URI:

https://boris.unibe.ch/id/eprint/179078

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